Sandwich enzyme immunoassay of osteocalcin in serum with use of an antibody against human osteocalcin
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منابع مشابه
Sandwich enzyme immunoassay of osteocalcin in serum with use of an antibody against human osteocalcin.
We have developed and thoroughly validated a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) on microtiter plates for osteocalcin in human serum with use of an antibody raised against human osteocalcin. We used a monoclonal antibody against bovine osteocalcin as the capture antibody; the second antibody was a polyclonal antibody against human osteocalcin. The amount of bound seco...
متن کاملAvidin-biotin enzyme immunoassay of osteocalcin in serum or plasma.
We describe a competitive enzyme immunoassay, the ExtrAvidin-biotin system, for determining osteocalcin in human serum or plasma. Antibodies were raised against bovine osteocalcin. Binding of the antibodies to osteocalcin was calcium-dependent. Limit of detection is 0.07 nmol/L (0.4 microgram/L). The standard curve for method is linear between 0.3 and 17.6 nmol/L (1.9 and 100 micrograms/L). Int...
متن کاملEvaluation of a bead-based enzyme immunoassay for the rapid detection of osteocalcin in human serum.
BACKGROUND Circulating osteocalcin is a well-known marker for bone formation, but none of the commercial kits currently available can be used in automated systems. Here we present the first semiautomated assay for human serum osteocalcin. METHODS Polystyrene beads were coated with antibodies against the COOH terminus of osteocalcin and used in the COBAS((R)) EIA System. Osteocalcin was detect...
متن کاملSolid-phase enzymoimmunoassay for osteocalcin in human serum or plasma, with use of a monoclonal antibody.
In this solid-phase enzymoimmunoassay on microtiter plates for osteocalcin in serum or plasma, we use an osteocalcin-horseradish-peroxidase conjugate and a monoclonal antibody raised against bovine osteocalcin. We thoroughly standardized the assay for measurement of osteocalcin in both serum and plasma, demonstrating independence of sample volume, and determining the analytical recovery and wit...
متن کاملA simple enzyme-linked immunosorbent assay of human osteocalcin.
A heterologous ELISA for measurement of osteocalcin (bone Gla-protein) is described, involving biotinylated bovine osteocalcin and polyclonal antibodies. The log-linear range was 2.3-37.5 micrograms/L. Between-run (total) and within-run CVs (n = 10) were 5.7-6.4 and 5.9-6.1%, respectively; analytical recoveries ranged from 92% to 108%. Comparison of our method (x) with an RIA (y) yielded y = 1....
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1993
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/39.6.942